Comment |
Fluo-2 MA is an ideal indicator for HTS and other calcium applications because of its easy loading, bright response, and lower cost compared to Fluo-4.
Fluo-2 MA (U.S. Patent No. 5,049,673) is the same molecule as Fluo-8.
Fluo-2 HA – High Affinty
Fluo-2 LA – Low Affinity
Fluo-2 LR- Leakage Resistant
Fluo-2 NM- Near Membrane
Fluo-2 Mito- Mitochondrial
Mag-Fluo-2- Magnesium
Leakage Resistant
These modified indicators are retained in the cell for hours, with little to no change in the properties of their parent compounds. Numerous publications have emanated since their inception in 1994.
Teflabs is the sole manufacturer of the leakage resistant dyes Fura-2 LR (Cat.0108, formerly Fura-PE3), Indo-1 LR (Cat. 0144, formerly Indo-PE3), Fluo-2 LR and Asante Calcium Red LR (Cat. 3150).
TEFLabs’ new sodium indicator, Asante Natrium, provides a low Kd, visible wavelength response to sodium ions. With an excitation maximum near the 488 nm argon laser line Asante Natrium shows a nearly five- fold increase in green emission upon saturating with sodium. The Kd of 7 mM is comparable to that of SBFI, in contrast to previous visible wavelength indicators, such as CoroNA Red, whose higher kd makes them unattractive for most cell applications.
Samples will be available for testing in mid-May.
Prices have been reduced for Indo-1 Am and 20 x 50.
Prices for Leakage Resistant (LR), Near Membrane (NM) and Low Affinity (LA) versions of Indo-1 and Fura-2 have also been reduced. Unit sizes have been increased to 1 mg breakdowns and prices for all versions have been standardized.
Fura-2 LR and Indo-1 LR (formerly Fura PE3 and Indo PE3), Fura-2 NM and Indo-1 NM (formerly FFP-18 and FIP-18), Fura-2 LA and Indo-1 LA (formerly Fura-2FF and Indo-1 FF)
AM 1 mg- $110
AM 20 x 50 ug- $130
K+Salt- $110
Order specialty Calcium Indicators
Fura-2
Fura-2 is a high affinity, UV excitable intracellular calcium indicator that exhibits the unique property of ratiometry, it is the most popular indicator to accurately quantify calcium. By applying the ratio of the two wavelengths, variable effects such as indicator concentration, degree of cell loading, photobleaching, detector sensitivity, cell thickness and optical path are all eliminated. Typically, Fura-2 is excited at 335 and 362 nm while monitoring the 505 nm emission. Fura-2 is available in the following forms:
Acetoxymethyl (AM) ester for non-invasive intracellular loading, >95% purity by HPLC
Potassium salt for calibration and invasive loading, >90% purity by HPLC
Teflabs will work to accommodate special requests; if you need a different form please let us know at probes@teflabs.com.
Order Fura-2
Want long wavelength ratiometry? Learn more about our new Asante Calcium Red
Special Application Variants of Fura-2
Teflabs also offers the low affinity, and near membrane specialty applications as well as leakage resistant enhanced-performance versions of Fura-2 and other calcium indicators.
Low Affinity
Low affinity Fura-2 has several advantages over its high affinity counterparts:
- Fluorescent response does not saturate at micromolar calcium levels
- Reduced buffering of intracellular calcium and reduced perturbation of calcium transients
- Faster dissociation from calcium allows for measurement of shorter-lived transients.
Our low affinity Fura-2 is based on the electron-withdrawing effect of fluorine on the affinity of the parent dye. Their Kds shift from the nanomolar to the micromolar range, with high selectivity for calcium over magnesium, unlike their APTRA based counterparts. We offer Fura-2 LA (Cat. 0135 formerly Fura-2 FF), Indo-1 LA (Cat. 0138 formerly Indo-1 FF), Fluo-2 LA, and Asante Calcium Red LA (Cat. 3050) low affinity indicators.
Leakage Resistant
These modified indicators are retained in the cell for hours, with little to no change in the properties of their parent compounds. Numerous publications have emanated since their inception in 1994.
Teflabs is the sole manufacturer of the leakage resistant dyes Fura-2 LR (Cat.0108, formerly Fura-PE3), Indo-1 LR (Cat. 0144, formerly Indo-PE3), Fluo-2 LR and Asante Calcium Red LR (Cat. 3150).
Near Membrane
The near-membrane calcium indicators continue to be highly successful in their niche applications. Unlike other hydrophobic indicators, these have a piperazine collar to latch into the membrane, leaving the chelating portion to measure calcium in the gates and not lost in the membrane. TEFLabs offers Fura-2 NM (Cat. 0125, formerly FFP-18), Indo-1 NM FIP-18(Cat. 0127, formerly FIP-18 ), Fluo-2 NM, and Asante Calcium Red NM (Cat. 3200 ).
TEFLabs offers both low affinity and near membrane specialty application versions of multiple calcium indicators.
Low Affinity
Low affinity calcium indicators have several advantages over their high affinity counterparts:
- Fluorescent response does not saturate at micromolar calcium levels
- Reduced buffering of intracellular calcium and reduced perturbation of calcium transients
- Faster dissociation from calcium allows for measurement of shorter-lived transients.
Our low affinity indicators are based on the electron-withdrawing effect of fluorine on the affinity of the parent dye. Their Kds shift from the nanomolar to the micromolar range, with high selectivity for calcium over magnesium, unlike their APTRA based counterparts. We offer Fura-2 LA (Cat. 0135 formerly Fura-2 FF), Indo-1 LA (Cat. 0138 formerly Indo-1 FF), Fluo-2 LA, and Asante Calcium Red LA (Cat. 3050) low affinity indicators.
Near Membrane
The near-membrane calcium indicators continue to be highly successful in their niche applications. Unlike other hydrophobic indicators, these have a piperazine collar to latch into the membrane, leaving the chelating portion to measure calcium in the gates and not lost in the membrane. TEFLabs offers Fura-2 NM (Cat. 0125, formerly FFP-18), Indo-1 NM FIP-18(Cat. 0127, formerly FIP-18 ), Fluo-2 NM, and Asante Calcium Red NM (Cat. 3200 ).
Asante Calcium Red, Fura-2, Fluo’s, Indo-1, and Rhod’s comparison based on Excitation, Emission, Ratiometry, Stokes, Photobleaching, and Loading characteristics.
Asante Calcium Red Dye Characteristic Comparison
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